Title: Green synthesis of silver nanoparticles using the plant extract of acer oblongifolium and study of Its antibacterial and antiproliferative activity via mathematical approaches

Abstract

In this study the antibacterial and antifungal properties of the silver nanoparticles synthesized with the aqueous plant extract of Acer oblongifolium leaves are defined using a simplistic, environmentally friendly, reliable, and cost-effective method. The aqueous plant extract of Acer oblongifolium, which served as a capping and reducing agent, was used to biosynthesize silver nanoparticles. UV visible spectroscopy, X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), and scanning electron microscopy were used to analyze the biosynthesized Acer oblongifolium silver nanoparticles (AgNPs). Gram-positive bacteria (Bacillus paramycoides and Bacillus cereus) and Gram-negative bacteria (E. coli) were used to test the AgNPs’ antibacterial activity. Presence of different functional groups were determined by FTIR. The AgNPs were rod-like in shape. The nanoparticles were more toxic against Escherichia coli than both Bacillus cereus and Bacillus paramycoides. The AgNPs had IC50 values of 6.22, 9.43 and mg/mL on HeLa and MCF-7, respectively, proving their comparatively strong potency against MCF-7. This confirmed that silver nanoparticles had strong antibacterial activity and antiproliferative ability against MCF-7 and HeLa cell lines. The mathematical modelling revealed that the pure nanoparticle has a high heat absorbing capacity compared to the mixed nanoparticle. This research demonstrated that the biosynthesized Acer oblongifolium AgNPs could be used as an antioxidant, antibacterial, and anticancer agent in the future.